Biotechnology

PRINCIPLES AND PROCESSES

A restriction enzyme called EcoRI from E.coli is expected to cleave the DNA at the following sequence:

GAATTC.(2001)

ANDI is the cloned:
Monkey.(2002)

The enzyme used to cut the DNA molecule is:
Restriction endonucleases.(2003)

The enzyme DNA polymerase was discovered by:
Kornberg.(2003)

Restriction endonuclease,an enzyme used in genetic engineering is employed for:
Cutting double stranded DNA.(2004)

Molecular scissors, which cut DNA at specific site:
Restriction endonucleases.(2004)

The technique used for separating molecules by passing electric fent:
Electrophoresis. ( 2004)

Who discovered recombinant DNA technology?:
Stanley Cohen and Herbert Boyer.(2007)

The enzyme used to join DNA fragments is:
DNA ligase.(2009)

Ti  plasmid used in genetic engineering is obtained from:
Agrobacterium tumefaciens.(2009)

APPLICATIONS

A cybrid is a hybrid carrying:
Cytoplasms of two different plants and genome of one plant.(2004)

What does Bt stand for in the popular crop Bt-cotton :
Bacillus thuringiensis.(2004)

SCID is caused by the defective gene coding for the enzyme called:
Adenosine deaminase.(2006)

The construction of the first rDNA was done by using the native plasmid of :
Salmonella typhimurium

CC -TIP 

Best of luck friends....